Composite

Part:BBa_K1632018

Designed by: Jun kawamura   Group: iGEM15_Tokyo_Tech   (2015-08-30)

J23100_rbs_lasR_TT_Plux_rbs_fimE(wild-type)

FimE is Fim recombinase.
FimE invert fim switch (example BBa_K1632000, BBa_K1632004 etc...) from the [ON] state to [OFF] state.
The expression of FimE is controlled by AHL in BBa_K1632018.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1034
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 927


[edit]
Categories
Parameters
None